Abstract
The primary cause of antibiotic use in dairy herds is mastitis, which requires microbiological identification for successful treatment and raises concerns due to increased antimicrobial resistance. This study aimed to highlight the profile of most bacterial species isolated from samples containing the β-lactamase gene from a dairy farm located in Dubai Emirate, UAE. We aseptically obtained a total of 83 milk samples from the herd of 9,000 Holstein milking cows that had clinically confirmed mastitis in March and April 2019. The real-time PCR and DNA extraction were done using the King Fisher™ Duo Prime Purification System, the Mag MAX™ CORE Nucleic Acid Purification Kit, and the VetMAX™ Masti Type Kit. PCR confirmed that 67 samples were positive, and the most common species found in samples with the β-lactamase gene were E. coli (N = 10), Staphylococci species (N = 15), and Streptococci species (N = 15), which were 14.93%, 22.39%, and 22.39% of all samples tested respectively. This result highlights the most bacterial species isolated throughout β-lactamase gene detection and presents their role in ß-lactam resistance.
Recommended Citation
Abdelhameed, Mohammed Farah Mohammed; Almansoori, Ahmed Eltigani; Ibrahim, Mohamed T.; Otaibi, Fatma Al; Ahmed, Louai; and Zayed, R. H.
(2025)
"Evaluating the Profile of Most Bacterial Species Isolated from Samples Containing β-lactamase Gene,"
Journal of Agricultural and Marine Sciences: Vol. 30:
Iss.
1, Article 7.
https://doi.org/10.53541/2410-1079.1347
Available at:
https://jams.squ.edu.om/home/vol30/iss1/7