Establishment and In Vitro Propagation of a Putative Variant of Periwinkle

Authors

A. S. AI-Wasel, Department of Horticulture and Forestry, College of Agriculture and Veterinary Medicine, King Saud University, P. 0. Box 1482, Al-Qassim, Saudi Arabia

Abstract

Shoot multiplication of a putative variant of Catharanthus roseus (L.) G. Don, was achieved in vitro using shoot tips and nodal segments as explants. The addition of growth regulators to establishment medium stimulated bud breaking and shoot elongation. The maximum shoot multiplication (15.1 shoots/microshoot) and the longest shoots (7.0 cm) occurred on Murashige and Skoog medium (MS) containing 1.0 mg L-1 of N6-Benzyladenine (BA) and a- Naphthalene acetic acid (NAA). All microshoots formed roots and normal root morphology occurred on half strength MS salt supplied with 0.5 mg L-1 NAA or Indole-B-Butyric acid (IBA). Rooted microshoots (95 %) were successfully transferred to soil.

Recommended Citation

AI-Wasel, A. S. (2000) "Establishment and In Vitro Propagation of a Putative Variant of Periwinkle," Journal of Agricultural and Marine Sciences: Vol. 5: Iss. 1, Article 6.
DOI: 10.24200/jams.vol5iss1pp15-18
Available at: https://jams.squ.edu.om/home/vol5/iss1/6

First Page

15

Last Page

18